Twist Bioscience Launches Multiplexed Gene Fragments to Enable High-throughput Screening Applications

Twist Bioscience Corporation (NASDAQ: TWST), a company enabling customers to succeed through its offering of high-quality synthetic DNA using its silicon platform, today announced the launch of Twist Multiplexed Gene Fragments (MGFs), pools of directly synthesized double-stranded DNA (dsDNA) up to 500 base pairs in length with no limit on sequence number to enable high throughput screening applications.

“Using our chemical synthesis platform we are able to directly synthesize Twist Multiplexed Gene Fragments as fragment pools of up to 500 bp in length. By offering Twist's high-quality gene fragments in a pooled format at prices that scale with the number of sequences, Twist is enabling customers to conduct applications that may have been previously unfeasible or cost-prohibitive, facilitating high-throughput screening in a way that is far more accessible to researchers across industry segments,” said Emily M. Leproust, Ph.D., CEO and co-founder of Twist Bioscience. “The capability of pooling directly synthesized fragments with high uniformity and representation allows for rapid screening of diverse targets for CRISPR and genome engineering, facilitates entire antibody variable coding regions for antibody discovery, and supports long coding stretches of protein variants for protein engineering applications.”

Twist MGFs are synthesized from customer-defined sequences up to 500 base pairs in length, generating an unlimited number of high-quality DNA fragments in parallel. Twist MGFs encompass a region large enough to span entire antibody variable domains and UTRs, providing customers with a purpose-built product to meet their needs. The expansive coding region also allows for customer customization and multifaceted design options. The pooled format of the fragments enables large-scale high-throughput screening.

Twist Multiplexed Gene Fragments (MGFs)

Twist Multiplexed Gene Fragments are pools of customizable double-stranded DNA (dsDNA) between 301-500 base pairs in length. Variant pool size starts at one thousand sequences, with no maximum. Twist MGFs have a turnaround time starting at 8 business days and can be easily integrated into a wide range of workflows for applications including prime editing, ultra-complex CRISPR-based functional screening, peptide and protein engineering, antibody discovery, mRNA vaccine development and massively parallel reporter assays (MPRA).